Studies on detection of nosema bombycis by hybridization in situ
2.
Selected one of the 14 strains - s93 , s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18 , then transformed into dh5 a . filtering the clone with hybridization in situ , a 1 kb frament clone has been cloned
3.
In this experiment , radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process . methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0 . 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al . thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum , and added to 50ml cell culture flasks
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